EMCARE™ Trials

Metabolic Measurements of Embryos

Analysis of pyruvate utilization by least-square analysis revealed a significant (P>0.001) increase in ¹⁴CO₂ production from embryo's stored in EHM (MOPS buffered EMCARE™ media) compared to OCM (Ovum Culture Media). Chi-square analysis of re-development to hatching also showed a significant (P>0.001) improvement in embryonic viability after storage in EHM at 25°C. Both ¹⁴CO₂ production and development after storage significantly decreased from 24 to 48h.

Embryo toxicity during development was assessed using in vitro-produced cattle embryos. It was concluded that a 10mM concentration is less effective than 20mM, zwitteronic buffers are better without CO₂ enrichment, and MOPS (buffer) was less toxic in terms of cleavage than either HEPES or BES (buffers). In fact, under MOPS, 20mM, no CO₂, 87% of embryo's reached the cleavage stage, whereas only 73% of HEPES, 20mM, no CO₂, reached cleavage. A difference of 19.2% in efficacy.

For more information refer to the EMCARE™ US Patent 5,716,847.